Peripheral amino acid appearance is lower following plant protein fibre products, compared to whey protein and fibre ingestion, in healthy older adults despite optimised amino acid profile

de Marco Castro, Elena ORCID: https://orcid.org/0000-0003-3581-2570, Valli, Giacomo ORCID: https://orcid.org/0000-0002-5221-580X, Buffière, Caroline, Guillet, Christelle, Mullen, Brian ORCID: https://orcid.org/0000-0002-3973-1105, Pratt, Jedd ORCID: https://orcid.org/0000-0002-7410-078X, Horner, Katy ORCID: https://orcid.org/0000-0002-5046-3658, Naumann-Gola, Susanne ORCID: https://orcid.org/0000-0001-6838-5574, Bader-Mittermaier, Stephanie, Paganini, Matteo, De Vito, Giuseppe ORCID: https://orcid.org/0000-0002-6855-9180, Roche, Helen M ORCID: https://orcid.org/0000-0002-0628-3318 and Dardevet, Dominic (2023) Peripheral amino acid appearance is lower following plant protein fibre products, compared to whey protein and fibre ingestion, in healthy older adults despite optimised amino acid profile. Nutrients, 15 (1). 35. ISSN 2072-6643

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Abstract

Plant-based proteins are generally characterised by lower Indispensable Amino Acid (IAA) content, digestibility, and anabolic properties, compared to animal-based proteins. However, they are environmentally friendlier, and wider consumption is advocated. Older adults have higher dietary protein needs to prevent sarcopenia, a disease marked by an accelerated loss of muscle mass and function. Given the lower environmental footprint of plant-based proteins and the importance of optimising dietary protein quality among older adults, this paper aims to assess the net peripheral Amino Acid (AA) appearance after ingestion of three different plant protein and fibre (PPF) products, compared to whey protein with added fibre (WPF), in healthy older adults. In a randomised, single-blind, crossover design, nine healthy men and women aged ≥65 years consumed four test meals balanced in AA according to the FAO reference protein for humans, matched for leucine, to optimally stimulate muscle protein synthesis in older adults. A fasted blood sample was drawn at each visit before consuming the test meal, followed by postprandial arterialise blood sampling every 30 min for 3 h. The test meal was composed of a soup containing either WPF or PPF 1–3. The PPF blends comprised pea proteins with varying additional rice, pumpkin, soy, oat, and/or almond protein. PPF product ingestion resulted in a lower maximal increase of postprandial leucine concentration and the sum of branched-chain AA (BCAA) and IAA concentrations, compared to WPF, with no effect on their incremental area under the curve. Plasma methionine and cysteine, and to a lesser extent threonine, appearance were limited after consuming the PPF products, but not WPF. Despite equal leucine doses, the WPF induced greater postprandial insulin concentrations than the PPF products. In conclusion, the postprandial appearance of AA is highly dependent on the protein source in older adults, despite providing equivalent IAA levels and dietary fibre. Coupled with lower insulin concentrations, this could imply less anabolic potential. Further investigation is required to understand the applicability of plant-based proteins in healthy older adults.